Institute for Stem Cell Biology and Regenerative Medicine

Renee Reijo Pera, Ph.D.
Principal Investigator
reneer@stanford.eduRenee Reijo Pera, Ph.D.

Dr. Reijo Pera is the Director of the Human Embryonic Stem Cell Research and Education and a Professor of Obstetrics and Gynecology at Stanford University. Her research is aimed at understanding the genetics of human embryo growth and development and in characterizing the basic properties of human embryonic stem cells, especially their ability to differentiate to all cell types including germ cells. She has received numerous awards throughout her career and most recently was named Outstanding Faculty Mentor at UCSF in 2005 and one of the twenty Top Women On Leadership featured in Newsweek 2006. She received her Bachelor of Science degree from the University of Wisconsin (Superior), her doctoral degree from Cornell University in Ithaca, New York, and her postdoctoral training at the Whitehead Institute for BioMedical Research at the Massachusetts Institute of Technology in Cambridge, Massachusetts.

Click here to see Dr. Reijo Pera's scientific publications

 

 

Erica Anderson
Life Science Research Assistant II
ericaa@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Cal Poly - San Luis Obispo, B.S. Cellular and Molecular Biology
San Jose State University, M.S. Biotechnology

Current Project:
Reprograming fibroblasts from UI patients and differentiating them to smooth muscle cells.

Interesting Facts/Hobbies:
I was born on an island in the Pacific Northwest, but I am now a California girl at heart. In my free time I love to read, go to shows, travel, and cook.

Publications:
Quarto N, Leonard B, Li S, Marchand M, Anderson E, Behr B, Francke U, Reijo-Pera R, Chiao E, Longaker MT. Skeletogenic phenotype of human Marfan embryonic stem cells faithfully phenocopied by patient-specific induced-pluripotent stem cells. Proc Natl Acad Sci USA. 2012;109:215–220.

Chung C, Anderson E, Reijo Pera R, Pruitt BL, Heilshorn SC. Hydrogel crosslinking density regulates temporal contractility of human embryonic stem cell-derived cardiomyocytes in 3D cultures. Soft Matter, 2012,8, 10141-10148.

 

 

Sharon Briggs
Graduate Student
sfbriggs@stanford.edu
Department of Genetics

Previous Affiliations:
Carnegie Mellon University, BS in Biological Sciences

Current Project:
Understanding the role of X chromosome reactivation during reprogramming to pluripotency. I am trying to determine if female cells reach a two active X chromosome state during the reprogramming process by using a time course approach.

Interesting Facts/Hobbies:
I am a big soccer player and general sports fan. I also love being in lab by myself so I can sing and dance in the TC room. I was born and raised in West Virginia and really miss the East Coast!

 

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Diana Cepeda
Research Associate
dianabio@gmail.com
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
University of British Columbia, Canada; B.S. in Cell Biology and Genetics
Uppsala University, Sweden; M.S in Molecular Biology
Karolinska Institute, Sweden; Ph.D. in Medical Sciences

Current Project:
Assembly of TALENs for genome editing.

Interesting Facts/Hobbies:
I love to travel and try different types of foods. I was born and raised in Colombia.

Publications:
Klotz K, Cepeda D, et al. SCF(Fbxw7/hCdc4) targets cyclin E2 for ubiquitindependent proteolysis. Experimental Cell Research, 2009;315(11):1832-9.

Sangfelt O, Cepeda D, et al. Both SCF(Cdc4alpha) and SCF(Cdc4gamma) are required for cyclin E turnover in cell lines that do not overexpress cyclin E. Cell required for cyclin E turnover in cell lines that do not overexpress cyclin E. Cell Cycle, 2008;7(8):1075-1082.

Akhoondi S, Sun D, von der Lehr N, Apostolidou S, Klotz K, Maljukova A, Cepeda D, et al. FBXW7/hCDC4 is a general tumor suppressor in human cancer. Cancer Research, 2007;67(19):9006-12.

Lerner M, Corcoran M, Cepeda D, et al. The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD. Molecular Biology of the Cell, 2007;18(5):1670-82.

 

 

 
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Shawn L. Chavez
Basic Life Science Research Associate
chavezs@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
University of California, Santa Barbara, BS in Biological Sciences
Yale University, PhD in Molecular, Cellular and Developmental Biology
University of California, San Francisco, Post-doctoral training

Current Project:
My research is focused on understanding the possible origins of human embryonic aneuploidy and the epigenetic regulation of early embryogenesis and germ cell differentiation.

Interesting Facts/Hobbies:
Enjoys running, gardening and almost all sports, but does not like leading us in a song.

Publications
Aschkenazi, S., Straszewski, S., Verwer, K., Foellmer, H., Rutherford, T., Mor, G. Differential regulation and function of the Fas/FasL system in human trophoblast cells. Biology of Reproduction 2002; 66: 1853-186.

Abrahams, V.M., Straszewski, S.L., Kamsteeg, M., Hanczaruk, B., Rutherford, T.J., Schwartz, P.E., Mor, G. Epithelial ovarian cancer cells secrete functional Fas ligand. Cancer Research 2003; 63: 5573-5581.

Straszewski-Chavez, S.L., Abrahams, V.M., Funai, E.F., Mor, G. X-linked inhibitor of apoptosis (XIAP) confers human trophoblast cell resistance to Fas-mediated apoptosis. Molecular Human Reproduction 2004; 10(1): 33-41.

Abrahams, V.M., Straszewski-Chavez, S.L., Guller, S., Mor, G. First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis. Molecular Human Reproduction 2004; 10(1): 55-63.

Abrahams, V.M., Bole-Aldo, P., Kim, Y.M., Straszewski-Chavez, S.L., Chaiworapongsa, T., Romero, R., Mor, G. Divergent trophoblast responses to bacterial products mediated by toll-like receptors. Journal of Immunology 2004; 173: 4286-4296.

Fest, S., Aldo, P.B., Abrahams, V. M., Visintin, I., Alvero, A., Chen, R., Chavez, S.L., Romero, R., Mor, G. Trophoblast–macrophage interactions: a regulatory network for the protection of pregnancy. American Journal of Reproductive Immunology 2007 57: 55–66.

Straszewski-Chavez, S.L., Visintin, I.P., Karassina, N., Los, G., Liston, P., Halaban, R., Mor, G.  XAF1 mediates TNF-a-induced apoptosis and XIAP cleavage by acting through the mitochondrial pathway. Journal of Biological Chemistry 2007 282(17): 13059-13072.

Chavez, S.L., Meneses, J.J., Nguyen, H.N., Kim, S.K. and Reijo Pera, R.A. Characterization of six new human embryonic stem cell lines (HSF-7, -8, -9. -10, -12 and -13) derived under minimal-animal component conditions. Stem Cells and Development 2008 17(3): 535-546.

Kossack, N., Meneses, J., Shefi, S., Nguyen, H.N., Chavez, S., Nicholas, C., Gromoll, J., Turek P.J., Reijo-Pera R.A. Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells. Stem Cells 2009 27(1): 138-149.

Straszewski-Chavez, S.L., Abrahams, V.M., Alvero, A.B., Aldo, P.B., Ma, Y., Guller, S., Romero, R., Mor, G. The isolation and characterization of a novel telomerase immortalized first trimester trophoblast cell line, Swan 71. Placenta 2009 30(11): 939-948.

Straszewski-Chavez, S.L., Abrahams, V.M., Aldo, P.B., Romero, R., Mor, G.  AKT controls first trimester trophoblast cell sensitivity to Fas-mediated apoptosis by regulating XIAP expression. Biology of Reproduction 2010 82(1): 146-152.

McElroy, S.L., Byrne, J.A., Chavez, S.L., Behr, B., Hsueh, A.J., Westphal, L.M., Reijo Pera, R.A. Parthenogenic blastocysts derived from cumulus-free in vitro matured human oocytes. PLoS ONE 2010 5(6): 10979.

Tang, C., Lee, A.S., Volkmer, J.P., Sahoo, D., Nag, D., Mosley, A.R., Inlay, M.A., Ardehali, R., Chavez, S.L., Reijo Pera, R.A., Behr, B., Wu, J.C., Weissman, I.L., Drukker, M. SSEA-5, an antibody defining a novel surface glycan on human pluripotent stem cells and its application to remove teratoma-forming cells as part of a surface antibody panel. Nature Biotechnology 2011 (29) 9: 829-834. 

Chavez, S.L., Loewke, K.E., Han, J., Moussavi, F., Colls, P., Munne, S., Behr, B., Reijo Pera, R.A. Dynmaic blastomere behaviour reflects human embryo ploidy by the four cell state. Nature Coomunications 2012 3:1251

 

 
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H. Rosaria Chiang
Postdoctoral Fellow
Rosaria@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Massachusetts Institute of Technology, Ph.D. in Biology;
Yale University, B.S. in Molecular Biophysics & Biochemistry, Economics;

Current Project:
Single-cell analysis of embryogenesis and germ cell differentiation

Interesting Facts/Hobbies:
I talk to my computer when I code… a lot.

Publications:
Shin C, Nam JW, Farh KK, Chiang HR, Shkumatava A, Bartel DP (2010) Expanding the microRNA targeting code: functional sites with centered pairing. Mol. Cell 38, 789-802.

Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP (2010) Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Genes Dev. 24, 992-1009.

Rao PK, Toyama Y, Chiang HR, Gupta S, Bauer M, Medvid R, Reinhardt F, Liao R, Krieger M, Jaenisch R, Lodish HF, Blelloch R (2009) Loss of cardiac microRNA-mediated regulation leads to dilated cardiomyopathy and heart failure. Circ. Res. 105, 585-594.

Grimson A, Srivastava M, Fahey B, Woodcroft BJ, Chiang HR, King N, Degnan BM, Rokhsar DS, Bartel DP (2008) Early origins and evolution of microRNAs and Piwi-interacting RNAs in animals. Nature 455, 1193-1197.

Batista PJ, Ruby JG, Claycomb JM, Chiang R, Fahlgren N, Kasschau KD, Chaves DA, Gu W, Vasale JJ, Duan S, Conte D Jr, Luo S, Schroth GP, Carrington JC, Bartel DP, Mello CC (2008) PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. Mol. Cell 31, 67-78.

Guarracino DA, Chiang HR, Banks TN, Lear JD, Hodsdon ME, Schepartz A (2006) Relationship between salt-bridge identity and 14-helix stability of beta3-peptides in aqueous buffer. Org. Lett. 8, 807-810.

 

   
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Margaret Cuadro
Administrative Assistant
mcuadro@stanford.edu

Previous Affiliations:
City College of San Francisco

Interesting Facts/Hobbies:
I was born and raised in San Francisco. I have three wonderful sons. And I love to cook.

 

 


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Jun Cui
Postdoctoral Fellow
juncui@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Cornell University, PhD in Genetics

Current Project:
Creation of stable cell line by genome editing methods; generating germ cell specific reporters

Interesting Facts/Hobbies:
reading, hiking, watching movies

Publications:
Sartain, C. V., Cui, J., Meisel, R. P., and Wolfner, M. F., (2011) The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development 138:1619-29.    

Cui, J., Sackton, K. L., Horner, V. L., Kumar, K. E., and Wolfner, M. F., (2008) Wispy, the Drosophila homolog of GLD-2, is required during oogenesis and egg activation. Genetics 178: 2017–2029.

 


   
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Pooja Desai
Undergraduate
pjdesai8@gmail.com
Department of Biology, Candidate for B.S. in Biological Sciences

Current Project:
Tracking the origin and maturation of hematopoetic stem cells (HSCs) through novel targeting methods.

Interesting Facts/Hobbies:
I miss the beach and my family in SoCal, but Stanford has won over my heart. Big fan of reading, running, eating out with friends, and Stanford football.

 


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Grace Do
Undergraduate Student
gracedo@stanford.edu
Biology Department (Undergraduate)

Current Project:
Epigenetic reprogramming of DNA methylation during human embryo development

Interesting Facts/Hobbies:
In my free time, I enjoy photography, reading good books, and hanging out with my friends. I'm originally from Chandler, AZ, and I have to say, I don't really miss the 110+ degree summers!

 


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Antonia Dominguez
Graduate Student
antonia@stanford.edu
Department of Genetics

Previous Affiliations
New Mexico State University

Current Project:
The goal of my project is to develop a system to identify genes located on the human X chromosome that require two copies for proper early development and germ cell development.

Interesting Facts/Hobbies:
When not in lab you can find me playing sand volleyball, climbing or running.

 

 

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Jens Durruthy-Durruthy
Graduate Student
durruthy@stanford.edu
Department of Medical Biotechnology

Previous Affiliations:
Technical University of Berlin

Current Project:
I primarily focus on the molecular mechanisms behind nuclear reprogramming. I use information obtained regarding normal human embryonic development and seek to shed light on molecule key events in the transition from a somatic differentiated cells into a pluripotent cell type. Also, reprogramming various cell types other than human fibroblasts is another focus.

Interesting Facts/Hobbies:
Even though I am from Germany I don't look like a typical German (probably because I was born in Cuba). I love sports, traveling and I do have a twin so watch out!

Publications:
www.stemgent.com :D

 

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Fang Fang
Postdoctoral Fellow
fang24@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
National University of Singapore, PhD in Computational and Systems Biology

Current Project:
Understanding transcriptional regulation during germ cell differentiation

Interesting Facts/Hobbies:
I like playing badminton, otherwise I am doing experiments, otherwise I am lying on the bed, sleeping…...


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Akshi Goyal
Life Science Research Associate
akshig@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Gurukul Kangri University, Masters in Chemistry
San Jose State University, Masters in Molecular Biology and Microbiology

Current Project:
A non-viral approach to study the role of epigenetic modifications in the reprogramming of human dermal fibroblasts to iPS cells

Interesting Facts/Hobbies:
I love cooking, painting and listening to music. I also love spending time with my family.

Publications:
Park J, Knezevich PL, Wung W, O'Hanlon SN, Goyal A, Benedetti KL, Barsi-Rhyne BJ, Raman M, Mock N, Bremer M, Vanhoven MK. A conserved juxtacrine signal regulates synaptic partner recognition in Caenorhabditis elegans. Neural Dev. 2011 Jun 10;6:28.


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Jenny Han (Jinnuo)
Postdoctoral Fellow
jinnuoh@stanford.edu
Department of Obstetrics and Gynecology

Previous Affiliations:
University of New South Wales, PhD in Stem cell biology

Current Project:
Generating a mouse model to study aneuploidy during embryo development.

Interesting Facts/Hobbies:
I joined Reijo Pera lab after finishing my M. Phil. at the University of Newcastle and PhD at the University of New South Wales, Australia, where I had a great experience. I enjoy listening to music, reading books, travelling the world and taking photographs (of both human beings and embryos).

 

Publications:
Niakan K, Han J, Pedersen RA, Simon C and Reijo Pera R. Human pre-implantation embryo development. Development. 2012 Mar; 1 (139): 829-841

Han J and Sidhu K. Reprogramming somatic cells by fusion with embryonic stem cells: present status of cell fusion and prospects in regenerative medicine. Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications. Atwood C, InTech Publisher; April 2011: 237-258.

Han J and Sidhu KS. Embryonic stem cell extracts: use in differentiation and reprogramming. Regen. Med. 2011 Mar; 6 (2): 215-227.

Han J, Sachdev P and Sidhu K. A combined epigenetic and non-genetic approach for reprogramming human somatic cells. PLoS ONE. 2010; 5(8): e12297.

Han J, Chung H, Sachdev P, Sidhu KS. Epigenetic modifications in reprogramming and lineage specifications from embryonic stem cells: current trends and relevance in developing regenerative medicine. Stem cells, regenerative medicine and Cancer. Singh SR, Nova Science Publishers; 2010.

Han J and Sidhu KS. Current concepts in reprogramming somatic cells to pluripotent state. Curr Stem Cell Res Ther. 2008 Jan; 3 (1):66-74.


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Amanpreet Kaur
Intern - San Jose State University
akaur5@yahoo.com
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations
University of California, Merced - B.S. Human Biology

Current Project:
My main research project involves reprogramming human fibroblasts into iPSCs on a single cell level using a non-virus induction method - modified mRNA.

Interesting Facts/Hobbies:
I love to sing and watch movies.


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Cynthia Klein
Lab Manager
cklein@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations
San Jose State, Hsueh Lab
OB/GYN Stanford University

Interesting Facts/Hobbies:
Love to Cook, Bake, travel and ride my motorcycle.

 

 

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Hsiao-lu Lee
Postdoctoral Fellow
hsiaolu@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Stanford University

Interesting Facts/Hobbies:
If I am not in lab, you will most likely find me dancing ( Argentine Tango), cooking, or teaching.

Publications:
Nakayama, N.; Wilson, S.; Stadelmann L., Lee, H.-L.; Cable, C.; Arumainayagam C.R. Low-Energy Electron Induced Chemistry of CF2Cl2: Implications for the Ozone Hole? J. Phys. Chem. B 2004, 108, 7950-7954.

Lord, S. J.; Lu, Z.; Wang, H.; Willets, K. A.; Schuck, P. J; Lee, H. -L.; Nishimura, S. Y.; Twieg, R. J.; Moerner, W. E. Photophysical Properties of Acene DCDHF Fluorophores: Long-Wavelength Single-Molecule Emitters Designed for Cellular Imaging. J. Phys. Chem. A  2007, 111, 89348941.

Lee, H.-L.; Flynn, N. T. X-ray Photoelectron Spectroscopy Chapter in Handbook of Applied Solid State Spectroscopy ; D. R. Vij, Ed.; Springer, 2006; pp 485507.

Lord, S. J.; Conley, N. R.; Lee, H.-L.; Nishimura, S. Y.; Pomerantz, A. K.; Willets, K. A.; Lu, Z.; Wang, H.; Liu, N.; Samuel, R.; Weber, R.; Semyonov, A.; He, M.; Twieg, R. J.; Moerner, W. E. Minireview: DCDHF Fluorophores for Single-Molecule Imaging in Cells. ChemPhysChem 2009, 10, 5565.

Lee, H.-L.; Dubikovskaya, E.A.; Hwang, H.; Semyonov, A.; Wang, H. ; Jones, L.R.; Twieg, R.J.; Moerner, W.E.; Wender, P.A. Single-Molecule Motions of Oligoarginine Transporter Conjugates on the Plasma Membrane of CHO Cells. J. Amer. Chem. Soc. 2008, 130, 9364-9370.

Lord, S. J.; Conley, N. R.; Lee, H.-L.; Samuel, R.; Liu, N.; Twieg, R. T.; Moerner, W. E. A Photoactivatable Push-Pull Fluorophore for Single-Molecule Imaging in Live Cells. J. Am. Chem. Soc. 2008, 130, 92049205.

Lord, S. J.; Conley, N. R.; Lee, H.-L.; Liu, N.; Samuel, R.; Twieg, R. J.; Moerner, W. E. Photoactivatable DCDHF fluorophores for single-molecule imaging. Proceedings. Proc. SPIE 2009, 7190, 719013.

Arumainayagam, C.R.; Lee, H.-L.; Nelson, R.; Haines, D. Low-Energy Electron-Induced Reactions in Condensed Matter. Surf. Sci. Rep., 2010, 65, 1-44.
Lord, S. J.; Lee, H.-L.; Moerner, W. E. Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells. Anal. Chem. 2010, 82, 2192-2203.

Lord, S. J.; Lee, H.-L.; Samuel, R.; Weber, R.; Liu, N.; Conley, N. R.; Thompson, M. A.; Twieg, R. J.; Moerner, W. E. Azido Push-Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels. J. Phys. Chem. B 2010, 114, 1415714167.

Lee, H.-L.; Lord, S. J.; Iwanaga, S.; Zhan, K.; Xie, H.; Williams, J. C.; Wang, H.; Bowman, G. R.; Goley, E. D.; Shapiro, L.; Twieg, R. J.; Rao, J.; Moerner W. E. Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores. J. Am. Chem. Soc. 2010, 132, 15099 15101.

Lee, H-L.; Sahl, S.; Lew, M.; Moerner, W.E.; The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision. Appl. Phys. Lett. 2012, 100, 153701

Byers, B.; Lee, H.-L.; Reijo Pera, R.; Modeling Parkinson's Disease Using Induced Pluripotent Stem Cells. Curr. Neurol. Neurosci. Rep., Curr Neurol Neurosci Rep. 2012 , 3, 237-42.

Ondrus*, A.; Lee*, H.-L.; Iwanaga, S.; Parsons, W.; Andresen, B.; Moerner, W.E.; Du Bois, J. (* =equal contributions); Fluorescent Saxitoxins for Live Cell Imaging of Single Voltage-Gated Sodium Ion Channels Beyond the Optical Diffraction Limit, Chemistry and Biology, 2012, 19,  902-912

 

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Smruti Phadnis
Postdoctoral Fellow
smruti@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations
Pune University, India (PhD in Biotechnology, BS and MS in Microbiology)

Current Project:
Stalking stem cells :-)

Interesting Facts/Hobbies:
Hiking, Traveling, Music, Cooking and Eating :-). Being 18 months old again while playing with my son ;-).

Publications:
Phadnis S.M., Joglekar M.V., Venkateshan V., Ghaskadbi S.M., Hardikar A.A., and Bhonde R.R. Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow–derived progenitor cells. In Vitro Cell Dev Biol Anim 42, 283 (2006)

Patki S.M., Kadam S.S., Phadnis S.M., Bhonde R.R. Who is the culprit for post menopausal syndrome? Uterus/Ovary! Med Hypotheses 71, 382 (2008)

Sahu S., Joglekar M.V., Dumbre R., Phadnis S.M., Tosh D., and Hardikar A.A. Islet-like cell clusters occur naturally in human gall bladder and are retained in diabetic conditions. Journal of Cellular and Molecular Medicine 13(5), 999 (2009)

Chandra V., Swetha G., Phadnis S.M., Nair P.D., and Bhonde R.R. Generation of Pancreatic Hormone expressing Islet like Cell Aggregates from Murine Adipose Tissue-derived Stem Cells. Stem Cells 27(8), 1941 (2009)

Maruwala P., Phadnis S.M., Bhonde R.R., and B. L. V. Prasad, In-situ synthesis of water dispersible bovine serum albumin capped gold and silver nanoparticles and their cytocompatibility studies. Colloids and Surfaces B: Biointerfaces 73(2), 224 (2009)

Phadnis S.M., Ghaskadbi S.M., Hardikar A.A., and Bhonde R.R. Mesenchymal stem cells derived from diabetic bone marrow portrait unique features reflecting influence of diabetic microenvironmental niche. The Review of Diabetic Studies 6(4), 260 (2009)

Swetha G, Chandra V., Phadnis S.M., and Bhonde R.R. Glomerular parietal epithelial cells of adult murine kidney undergo EMT to generate cells with traits of renal progenitors. Journal of Cellular and Molecular Medicine 15(2), 396 (2011)

Phadnis S.M., Joglekar M.V., Dalvi M.P Muthyala S., Nair P.D., Ghaskadbi S.M., Bhonde R.R. and Hardikar A.A. Human bone marrow-derived mesenchymal cells differentiate and mature to into pancreatic lineage in vivo. Cytotherapy 13(3), 279 (2011)

 

 
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Cyril Ramanthal
Postdoctoral Fellow
cyrilr@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
University of Illinois Urbana-Champaign

Current Project:
The goal of my research is to explore the requirement of the AZFc (Azoospermia Factor C) genes for human sperm production. It is believed that the functions of the genes expressed from this large (5-10 MB) region of the Y chromosome are highly important for normal sperm production. I am utilizing reprogramming strategies to generate patient-specific pluripotent stem cells from infertile men who carry deletions of the AZFc genes and derive germ cell precursors (PGC-like cells) from these cells using genetic approaches. Using genetic complementation analysis, I will try to dissect the requirement of AZFc genes for normal sperm production and function. The intention of this strategy is twofold: First, to recover normal sperm production from the somatic skin cells of patients who carry an AZFc deletion and cannot make their own sperm. Second, to design and implement a strategy that may translate to other models of gene-based human pathologies wherein patient-derived cells can be reprogrammed and genetically altered for use in human developmental genetic analysis.

Interesting Facts/Hobbies:
Playing Soccer, Big sports fan in general. Im also an avid fan for new music, preferably house music. I grew up in East Africa and then moved to my home country India before coming to the US in 2000 to start my undergraduate program.

Publications:
Medrano JV, Ramathal C, Nguyen HN, Simon C, Reijo Pera RA. Divergent
RNA-binding proteins, DAZL and VASA, induce meiotic progression in human germcells derived in vitro. Stem Cells. 2012 Mar;30(3):441-51.

Ramathal C, Wang W, Hunt E, Bagchi IC, Bagchi MK. Transcription factor CCAAT enhancer-binding protein beta (C/EBPbeta) regulates the formation of a unique extracellular matrix that controls uterine stromal differentiation and embryo implantation. J Biol Chem. 2011 Jun 3;286(22):19860-71.

Ramathal CY, Bagchi IC, Taylor RN, Bagchi MK. Endometrial decidualization: of
mice and men. Semin Reprod Med. 2010 Jan;28(1):17-26.

Ramathal C, Bagchi IC, Bagchi MK. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol. 2010 Apr;30(7):1607-19.

 

   
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Sonya Schuh-Huerta
Postdoctoral Fellow
sonyas1@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:

University of Washington, Ph.D. in Physiology & Biophysics
Humboldt State University, B.S. in Marine Biology and Zoology

Current Project:
I have great interests in reproductive genetics and developmental biology.  My research focus is on the genes and molecular pathways of gamete development and physiology, with underlying goals of translational and clinical applications for human infertility treatments and conservation biology.  I have investigated the genes, variants, and environmental factors associated with ovarian follicle (egg) number, ovarian reserve, follicle loss and diminished fertility, and reproductive lifespan in women.  Other recent studies are using cell- and tissue-based approaches to investigate the roles of some of these genes in germ cell, gamete, and early embryo development in the human and model organisms.

Interesting Facts/Hobbies:
I love many different sports – swimming, cycling, running, kickboxing, Muay Thai, dancing, etc.  Over this past 2 years I have competed in over 7 Triathalons and Duathalons (most with my daughter).  I enjoy doing art of many forms including drawing, painting, and jewelry-making.  I also love teaching.  I was born and raised in Southern California and my parents have both been teachers.  I strongly believe that hard work and perseverance are more important than innate ability, intelligence, or anything else – and with these any goal can be achieved.  My favorite quote:  "Art is the Queen of all sciences communicating knowledge to all the generations of the world."  -Leonardo Da Vinci

Publications:
Krasnow SM, Fraley GS, Schuh SM, Baumgartner, JW, Clifton DK, Steiner RA (2003).  A role for galanin-like peptide in the integration of feeding, body weight regulation, and reproduction in the mouse.  Endocrinology, 144(3):813-822.

Carlson AE, Quill TA, Westenbroek RE, Schuh, SM, Hille B, Babcock DF (2005).  Identical phenotypes of CatSper1 and CatSper2 null sperm.  J Biol Chem, 280(37):32238-32244.

Schuh, SM, Carlson AE, McKnight GS, Conti M, Hille B, Babcock DF (2006).  Signaling pathways for modulation of mouse sperm motility by adenosine and catecholamine agonists.  Biol Reprod, 74:492-500.

Xie F, Garcia MA, Carlson AE, Schuh SM, Babcock DF, Jaiswal BS, Gossen J, Esposito G, van Duin M, Conti M (2006).  Soluble adenylyl cyclase (sAC) is indispensable for sperm function and fertilizationDev Biol, 396(2):353-362.

Schuh SM (2007).  Signaling pathways of mammalian sperm capacitation.  Ph.D. Dissertation, University of  
Washington, 141 pages (UMI No. AAT 3265407), Dissertations and Theses Database.

Schuh SM, Hille B, Babcock DF (2007).  Adenosine and catecholamine agonists speed the flagellar beat of mammalian sperm by a non-receptor-mediated mechanismBiol Reprod, 77(6):960-969.

Rosen MP, Sternfeld B, Schuh-Huerta SM, Reijo Pera RA, McCulloch CE, Cedars MI (2010).  Antral Follicle Count – absence of significant midlife declineFertil Steril, 94(6):2182-2185.

Schuh-Huerta SM and Reijo Pera RA (2010).  Making germ cells from human embryonic stem cells.  In: Male Germline Stem Cells: Developmental and Regenerative Potential (eds. K Orwig, B Hermann), pp 49-86, Humana Press, New York. ISBN 978-1-61737-972-7.

Schuh-Huerta SM and Reijo Pera RA (2011).  Reproductive Biology: Bone returns the favor.  Nature, 472:46-47.

Schuh-Huerta SM, Johnson NA, Rosen MP, Sternfeld B, Cedars MI, Reijo Pera RA (2012).  Genetic and environmental factors associated with hormonal markers of ovarian reserve in Caucasian and African American womenHum Reprod, 27:594-608.

Rosen MP, Johnstone E, McCulloch CE, Schuh-Huerta SM, Sternfeld B, Reijo Pera RA, Cedars MI (2012).  A characterization of the relationship of ovarian reserve markers with ageFertil Steril, 97(1):238-43.

Schuh-Huerta SM, Johnson NA, Rosen MP, Sternfeld B, Cedars MI, Reijo Pera RA (2012).  Genetic markers of ovarian follicle number and menopause in women of multiple ethnicitiesHum Genet, in press, doi:10.1007/s00439-012-1184-0.

 


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Vittorio Sebastiano
Instructor, Director of the hESCs Facility
vsebast@stanford.edu
OBGyN

Previous Affiliations:
Max Planck Institute for Molecular Biomedicine

Current Project:
Developing in-vivo modeling to study differentiation and human fetal development

Interesting Facts/Hobbies:

Publications:
Ge X, Wang IN, Toma I, Sebastiano V, Liu J, Butte MJ, Reijo Pera RA, Yang PC. Human Amniotic Mesenchymal Stem Cell-Derived Induced Pluripotent Stem Cells May Generate a Universal Source of Cardiac Cells. Stem Cells Dev. 2012 Jun 11. [Epub ahead of print]

Sebastiano V, Maeder ML, Angstman JF, Haddad B, Khayter C, Yeo DT, Goodwin MJ, Hawkins JS, Ramirez CL, Batista LF, Artandi SE, Wernig M, Joung JK (2011) In Situ Genetic Correction of the Sickle Cell Anemia Mutation in Human Induced Pluripotent Stem Cells Using Engineered Zinc Finger Nucleases. Stem Cells 29:1717-26.

Psathaki OE, Hubner K, Sabour D, Sebastiano V, Wu G, Sugawa F, Wieacker P, Pennekamp P, Scholer HR (2011) Ultrastructural Characterization of Mouse Embryonic Stem Cell-Derived Oocytes and Granulosa Cells. Stem Cells Dev 20: 2205-15.

Pang ZP, Yang N, Vierbuchen T, Ostermeier A, Fuentes DR, Yang TQ, Citri A, Sebastiano V, Marro S, Sudhof TC, Wernig M (2011) Induction of human neuronal cells by defined transcription factors. Nature 476: 220-223

Imberti B, Casiraghi F, Cugini D, Azzollini N, Cassis P, Todeschini M, Solini S, Sebastiano V, Zuccotti M, Garagna S, Redi CA, Noris M, Morigi M, Remuzzi G (2011) Embryonic stem cells, derived either after in vitro fertilization or nuclear transfer, prolong survival of semiallogeneic heart transplants. J Immunol 186: 4164-4174

Batista LF, Pech MF, Zhong FL, Nguyen HN, Xie KT, Zaug AJ, Crary SM, Choi J, Sebastiano V, Cherry A, Giri N, Wernig M, Alter BP, Cech TR, Savage SA, Reijo Pera RA, Artandi SE (2011) Telomere shortening and loss of self-renewal in dyskeratosis congenita induced pluripotent stem cells. Nature 474: 399-402

Wossidlo M, Arand J, Sebastiano V, Lepikhov K, Boiani M, Reinhardt R, Scholer H, Walter J (2010) Dynamic link of DNA demethylation, DNA strand breaks and repair in mouse zygotes. Embo J 29: 1877-1888

Sebastiano V, Dalvai M, Gentile L, Schubart K, Sutter J, Wu GM, Tapia N, Esch D, Ju JY, Hubner K, Bravo MJ, Scholer HR, Cavaleri F, Matthias P (2010) Oct1 regulates trophoblast development during early mouse embryogenesis. Development 137: 3551-3560

Ko K, Tapia N, Wu G, Kim JB, Bravo MJ, Sasse P, Glaser T, Ruau D, Han DW, Greber B, Hausdorfer K, Sebastiano V, Stehling M, Fleischmann BK, Brustle O, Zenke M, Scholer HR (2009) Induction of pluripotency in adult unipotent germline stem cells. Cell Stem Cell 5: 87-96

Kim JB, Sebastiano V, Wu G, Arauzo-Bravo MJ, Sasse P, Gentile L, Ko K, Ruau D, Ehrich M, van den Boom D, Meyer J, Hubner K, Bernemann C, Ortmeier C, Zenke M, Fleischmann BK, Zaehres H, Scholer HR (2009) Oct4-induced pluripotency in adult neural stem cells. Cell 136: 411-419

Kim JB, Zaehres H, Wu G, Gentile L, Ko K, Sebastiano V, Arauzo-Bravo MJ, Ruau D, Han DW, Zenke M, Scholer HR (2008) Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors. Nature 454: 646-650

Zuccotti M, Sebastiano V, Garagna S, Redi CA (2005) Experimental demonstration that mammalian oocytes are not selective towards X- or Y-bearing sperm. Mol Reprod Dev 71: 245-246

Sebastiano V, Gentile L, Garagna S, Redi CA, Zuccotti M (2005) Cloned pre-implantation mouse embryos show correct timing but altered levels of gene expression. Mol Reprod Dev 70: 146-154

Gentile L, Monti M, Sebastiano V, Merico V, Nicolai R, Calvani M, Garagna S, Redi CA, Zuccotti M (2004) Single-cell quantitative RT-PCR analysis of Cpt1b and Cpt2 gene expression in mouse antral oocytes and in preimplantation embryos. Cytogenet Genome Res 105: 215-221

Garagna S, Merico V, Sebastiano V, Monti M, Orlandini G, Gatti R, Scandroglio R, Redi CA, Zuccotti M (2004) Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis. J Mol Histol 35: 631-638


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Maria Vera Rodriguez
Visiting Student Researcher
mverrod@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Pablo de Olavide University, B.S. in Biotechnology
Valencia University, M.S. in Biotechnology of Assisted Human Reproduction

Current Project:
Studying fragmentation, its origin and consequences during human embryo development.

Interesting Facts/Hobbies:
Taking pictures, feeling the music, enjoying nature, getting hooked on a book, being walked by my dog ??and making someone laugh.

Publications:
Vera, M., Peinado, V., Al-Asmar, N., Gruhn, J., Rodrigo, L., Hassold, T., Rubio, C. (2012)Human male meiosis and sperm aneuploidies, In: Aneuploidy in health and disease, Intech,

Al-Asmar, N., Peinado, V., Vera, M., Remohí, J., Pellicer, A., Simón, C., Hassold, T., Rubio, C. (2012) Chromosomal abnormalities in embryos from couples with a previous aneuploid miscarriage. Fertil. Steril. 2012 Apr 20.

Mundi, M., Moreno, J.M., Quiroga, R., Vera, M., Garrido, N., Labarta, E. (2010) Review of the effect of controlled ovarian stimulation on the incidence of aneuploidy. Revista Iberoamericana de fertilidad.

 


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Prachi Wani
Life Science Research Assistant
pgujar@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
San Jose State University, Masters of Biotechnology
Pune University, BS Microbiology & MS Public Health

Current Project:
Working with disease specific iPSCs to model Parkinson's Disease.

Interesting Facts/Hobbies:
Coming from the land where different cultures Harbor. I like to travel, watch movies, cook various cuisines , spicy foods being the most favorite and spend time with my family.


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Mark Wossidlo
Postdoctoral Fellow
wossidlo@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Department of EpiGenetics, University of Saarbruecken, Germany

Current Project:
I am interested in the epigenetic reprogramming of DNA methylation during early mammalian preimplantation development. Early mammalian development is characterized by dramatic epigenetic changes. Upon fertilization of the oocyte with the sperm, the maternal and paternal genomes of the zygote are extensively reprogrammed to ensure the development of a totipotent potential. During this period of epigenetic reprogramming, DNA methylation (5-methyl-cytosine, 5mC) of paternal and maternal chromosomes is erased and reset during formation of the blastocyst. Interestingly, in mouse zygotes, the paternal genome becomes actively demethylated, as judged by immunofluorescence with antibodies against 5mC and bisulfite-sequencing data. Since the discovery of active DNA demethylation many scientists were trying to identify the putative “DNA demethylase” and a lot of candidate enzymes and pathways have been suggested and disproven. The identification of the enzymatic conversion of 5mC to 5-hydroxymethyl-cytosine (5hmC), 5-formyl-cytosine (5fC) and 5-carboxyl-cytosine (5caC) by Tet1-3 enzymes sheds new light on this process.
However, the analysis of epigenetic reprogramming in mammals is mainly focused on the mouse model and little is known about human embryonic development. Understanding the basic molecular mechanisms of human epigenetic reprogramming will impact human reproductive health and the generation of pluripotent stem cells.

Interesting Facts/Hobbies:
I am a very outdoorsy person and like to do all kinds of sports and games also in a competitive way ;) If I am not in the lab I climb mountains, play beach volleyball or enjoy the ocean..

Publications:
Lange UC, Siebert S, Wossidlo M, Weiss T, Zeigler-Birling C, Walter J, Torres-Padilla ME, Daujat S, Schneider R. 2013 Dissecting the role of H3K64me3 in mouse pericentromeric heterochromatin. Nat. Commun.

Wossidlo M, Nakamura T, Lepikhov K, Marques C.J, Zakhartchenko V, Boiani M, Arand J, Nakano T, Reik W, Walter J. 2011. 5-hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. Nat. Commun.

Lepikhov K, Arand J, Wossidlo M, Walter J. 2011. Epigenetic Reprogramming in Mammalian Development. EMBBM

Lepikhov K, Wossidlo M, Arand J, Walter J. 2010. DNA methylation reprogramming and DNA repair in the mouse zygote. Int. J. Dev. Biol. 54: 1565-1574

Arand J, Lepikhov K, Wossidlo M, Walter J. 2010 Active DNA demethylation - the enigma starts in the zygote. Epigenomics: From Chromatin Biology to Therapeutics. Book chapter

Wossidlo M, Arand J, Sebastiano V, Lepikhov K, Boiani M, Reinhardt R, Scholer H, Walter J. 2010. Dynamic link of DNA demethylation, DNA strand breaks and repair in mouse zygotes. Embo J 29: 1877-1888


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Pengbo Zhang
Postdoctoral Fellow
zhangpb@stanford.edu
Institute for Stem Cell Biology and Regenerative Medicine

Previous Affiliations:
Peking University, Beijing, P.R. China

Current Project:
Modeling Parkinson's disease using patient iPS cell-derived dopaminergic neurons.

Interesting Facts/Hobbies:
I love playing table tennis and badminton.

Publications:
Wang C, Faloon PW, Tan Z, Lv Y, Zhang P, Ge Y, Deng H, Xiong JW. Mouse lysocardiolipin acyltransferase controls the development of hematopoietic and endothelial lineages during in vitro embryonic stem-cell differentiation. Blood, 2007 Nov 15; 110(10):3601-9.

Zhang P*, Li J*, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, Du L, Ding M, Deng H. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood, 2008 Feb 15; 111(4):1933-41.

Zhou H*, Yong J*, Sun X*, Wang C, Yang W, Zhang P, Zhu J, Shi C, Ding M, Deng H. A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells. Differentiation, 2008 Nov; 76(9):923-30.

Zhao Y*, Yin X*, Qin H*, Zhu F?, Liu H?, Yang W?, Zhang Q?, Xiang C?, Hou P?, Song Z, Liu Y, Yong J, Zhang P, Cai J, Liu M, Li H, Li Y, Qu X, Cui K, Zhang W, Xiang T, Wu Y, Zhao Y, Liu C, Yu C, Yuan K, Lou J, Ding M, Deng H. Two supporting factors greatly improve the efficiency of human iPSC generation. Cell Stem Cell, 2008 Nov 6; 3(5):475-9.

Liu H*, Zhu F*, Yong J*, Zhang P, Hou P, Li H, Jiang W, Cai J, Liu M, Cui K, Qu X, Xiang T, Lu D, Chi X, Gao G, Ji W, Ding M, Deng H. Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts. Cell Stem Cell, 2008 Dec 4; 3(6):587-90.

Zhu FF*, Zhang PB*, Zhang DH*, Sui X, Yin M, Xiang TT, Shi Y, Ding MX, Deng H. Generation of pancreatic insulin-producing cells from rhesus monkey induced pluripotent stem cells. Diabetologia, 2011 Sep;54(9):2325-36.

Wang C*, Tang X*, Sun X*, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, Du L, Gao Y, Yin M, Ding M, Deng H. TGFβ inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy. Cell Res. 2012 Jan;22(1):194-207.

 

 

 

 

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